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Proteins, Expression, Isolation and Analysis | … For Immunoprecipitation, lysates were incubated with the anti-O-GlcNAC antibody (CTD110.6 and RL2) overnight at 4C and then bound to protein A/G sepharose beads (Genscript; Piscataway, NJ, USA), washed, and then separated using SDS-PAGE … | Get A Quote |
Idiopathic pulmonary arterial hypertension (IPAH) is a progressive and devastating disease characterized by vascular smooth muscle and endothelial cell proliferation leading to a narrowing of the vessels in the lung. The increased resistance in the lung and the higher pressures generated result in right heart failure. Nitric Oxide (NO) deficiency is considered a hallmark of IPAH and altered function of endothelial nitric oxide synthase (eNOS), decreases NO production. We recently demonstrated that glucose dysregulation results in augmented protein serine/threonine hydroxyl-linked N-Acetyl-glucosamine (O-GlcNAc) modification in IPAH. In diabetes, dysregulated glucose metabolism has been shown to regulate eNOS fu... More