| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … At present, it is uncertain whether N-specific antibodies are protective against CoV-2, but for murine coronavirus it has been demonstrated … was achieved by sequencing the variable regions of KL53, synthesising cDNA encod- ing the heavy and light chains (Genscript Inc, USA … | Get A Quote |
Nucleoprotein (N) is an immunodominant antigen in many enveloped virus infections. While the diagnostic value of anti-N antibodies is clear, their role in immunity is not. This is because while they are non-neutralising, they somehow clear infection by coronavirus, influenza and LCMV in vivo. Here, we show that anti-N immune protection is mediated by the cytosolic Fc receptor and E3 ubiquitin ligase TRIM21. Exploiting LCMV as a model system, we demonstrate that TRIM21 uses anti-N antibodies to target N for cytosolic degradation and generate cytotoxic T cells (CTLs) against N peptide. These CTLs rapidly eliminate N-peptide-displaying cells and drive efficient viral clearance. These results reveal a new mechanism... More
