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Proteins, Expression, Isolation and Analysis | … The sequence coding for the human TGF-β1 (hTGF-β1) containing 6×His (His tag) at the C terminus was synthesized by GenScript Corporation (NJ, USA), which was optimized by employing codons that are preferentially used in genes encoding major rice seed storage proteins … | Get A Quote |
Human transforming growth factor-β1 (hTGF-β1) was produced in transgenic rice seeds. To boost its production yield and to extract it simply, it was expressed under the control of seed-specific promoters along with the simultaneous suppression of endogenous seed storage proteins (SSPs) through RNA interference (RNAi). When driven by the 26 kDa α-globulin endosperm-specific promoter, it accumulated up to the markedly high level of 452 μg/grain. However, exchange with other seed-specific promoters such as 18 kDa oleosin and AGPase promoters resulted in remarkable reduction to the levels of 62 and 48 μg/grain, respectively, even though endogenous SSPs were reduced to the similar level. These production l... More