| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … PCR was performed on ABI 7500 Real-Time PCR System and the relative gene expression was analyzed using the ΔΔCt method, GAPDH was used as an internal control. The primers were synthesized by GenScript (Najing, China) and the sequences were listed in Table 1 … | Get A Quote |
Glioblastoma is the most common and malignant tumor in human central nervous system with poor prognosis. From the dried stem of , an herbal medicine, five compounds (sinomenine, syringin, corchoionoside C, protocatechuic acid and cannabisin D) were isolated, characterized and subjected to cytotoxicity screening on U-87 and U-251 glioblastoma cells. Cannabisin D presented effective inhibitory effects on the proliferation and migration of glioblastoma cells. By flow cytometry, real-time PCR and Western blotting, cell apoptosis and cell cycle arrest were proved to contribute to the anti-glioblastoma effects. Further, the activation of MAPKs signaling (p38 MAPK, p42/p44 MAPK and SAPK/JNK) was observed in glioblasto... More
