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Isoquercitrin induces apoptosis and autophagy in hepatocellular carcinoma cells via AMPK/mTOR/p70S6K signaling pathway

Aging (Albany NY). 2020-11; 
Liyan Shui, Weina Wang, Mingjie Xie, Bingjue Ye, Xian Li, Yanning Liu, Min Zheng
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Molecular Biology Reagents … HepG2 and Huh7cells were treated with 400 μM ISO for 48 h in the presence or absence of 100 nM rapamycin (RAPA) or 100 … Protein samples (30 μg) were subjected to 4-20% gradient PAGE gel (GenScript M42012) with Tris- MOPS running buffer, and transferred onto PVDF … Get A Quote

摘要

Hepatocellular carcinoma (HCC) is an aggressive malignancy with high rates of metastasis and relapse. Isoquercitrin (ISO), a natural flavonoid present in the Chinese bayberry and other plant species, reportedly exerts notable inhibitory effects on tumor cell proliferation, though the mechanism is unknown. In the present study, we exposed HepG2 and Huh7 human liver cancer cells to ISO and examined the roles of autophagy and apoptosis in ISO-mediated cell death. We found that ISO exposure inhibited cell viability and colony growth, activated apoptotic pathway, and triggered dysregulated autophagy by activating the AMPK/mTOR/p70S6K pathway. Autophagy inhibition using 3-methyladenine (3-MA) or Atg5-targeted siRNA d... More

关键词

AMPK, apoptosis, autophagy, hepatocellular carcinoma, isoquercitrin