Functional analysis of an R311C variant of Ca2+ -calmodulin-dependent protein kinase kinase-2 (CaMKK2) found as a de novo mutation in a patient with bipolar disorder.
| Products/Services Used |
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Operation |
| Peptide Synthesis |
02% [v/v] Brij-35) containing 200 µmol L-1 CaMKKtide (synthesized by Genscript , Piscataway, NJ, USA), 100 µmol L-1 CaCl2, 0–1 µmol L-1 CaM (Sigma-Aldrich), 200 µmol L-1 [γ-32P]-ATP (Perkin Elmer, G len Waverley, V ictoria, Australia) and 5 mmol L-1 MgCl2 (Sigma-Aldrich) in a standard 30 µl assay for 10 min at 30°C |
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