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Proteins, Expression, Isolation and Analysis | … WB to detect gephyrin phosphorylation was performed using either anti-phospho-gephyrin Ser-303 (1 µg/ml, custom made by Genscript) or Ser-305 (1mg/mL, custom made by Genscript) and commassie stain to detect total gephyrin. Western blots … | Get A Quote |
Synaptic plasticity relies on the rapid changes in neurotransmitter receptor number at postsynaptic sites. Using superresolution photoactivatable localization microscopy imaging and quantum dot-based single-particle tracking in rat hippocampal cultured neurons, we investigated whether the phosphorylation status of the main scaffolding protein gephyrin influenced the organization of the gephyrin scaffold and GABA receptor (GABAR) membrane dynamics. We found that gephyrin phosphorylation regulates gephyrin microdomain compaction. Extracellular signal-regulated kinase 1/2 and glycogen synthase kinase 3β (GSK3β) signaling alter the gephyrin scaffold mesh differentially. Differences in scaffold organization simila... More