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Growth Response of Lactobacillus rhamnosus Chloramphenicol-Resistant Strain ATCC 27773 to Pteroyl-Mono- and -Di-Glutamates

J Nutr Sci Vitaminol (Tokyo). 2019;

Kyohei Koseki, Naho Okamoto, Tomohiro Bito, Shuhei Ebara, Yukinori Yabuta, Fumio Watanabe

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Codon Optimization	… fdc1 gene (genbank accession number NM — 001180847.1) and yeast pad1 gene (genbank accession number NM — 001180846) that were codon optimized for expression in E. coli (SEQ ID NOS: 3 & 4, respectively) were commercially obtained from GenScript and provided …	Get A Quote

摘要

This study investigated the effect of various concentrations of pteroyl-mono-γ-glutamate (PteGlu) and pteroyl-di-γ-glutamate (PteGlu) on the growth of Lactobacillus rhamnosus strains ATCC 7469 (wild-type strain) and ATCC 27773 (chloramphenicol-resistant strain) used for folate microbiological assays. At concentrations of 0.025-0.20 nmol/L, the growth of the chloramphenicol-resistant strain was stimulated to a greater extent by PteGlu than by PteGlu, but the wild-type strain did not show such phenomena. L. rhamnosus ATCC 27773 bioassays were used to determine the total folate content of various foods treated with a chicken pancreas folate conjugase. This showed a significantly lower value when PteGlu was used ... More