Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis | … The membrane was incubated with THE His tag antibody (Genscript) according to the One Hour Western protocol (Genscript) and developed using LumiSensor (Genscript) chemiluminescent HRP substrate detection in a Fuji LAS-1000plus CCD camera. Purification of LciA … | Get A Quote |
The class IId bacteriocin lactococcin A and the pediocin-like bacteriocins induce membrane leakage and cell death by specifically binding the mannose phophotransferase system (man-PTS) on their target cells. The bacteriocins' cognate immunity proteins that protect the producer cell from its own bacteriocin recognize and bind to the bacteriocin-man-PTS complex and thereby block membrane leakage. In this study, we have determined the three-dimensional structure of the lactococcin A immunity protein (LciA) by the use of nuclear magnetic resonance spectroscopy. LciA forms a four-helix bundle structure with a flexible C-terminal tail. Despite the low degree of sequence similarity between LciA and the pediocin-like i... More