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Internal standard fluorogenic probe based on vibration-induced emission for visualizing PTP1B in living cells

Chem Commun (Camb). 2019-12-01;

Qiuyu Gong, Wenjing Qin, Peng Xiao, Xiang Wu, Lin Li, Gaobin Zhang, Renshuai Zhang, Jinpeng Sun, Shao Q Yao, Wei Huang

Products/Services Used	Details	Operation
Peptide Synthesis	… EXAMPLES Methods and Materials; [0051]. 1. Reagents. [0052]. All synthetic peptides are from GenScript. TAMRA-6-maleimide is from InVitrogen. Amylose agarose resin is from New England Biolabs. Ni-NTA agarose and proteinase K (>600 mAU/ml) are from Qiagen …	Get A Quote

摘要

Herein, as a proof of concept, we developed the first enzymatic VIE fluorogenic probe for protein tyrosine phosphatase 1B (PTP1B). The detection and imaging of PTP1B using VIE in living cells were both realized. Particularly importantly, the designed probe herein provides a guideline and platform for the development of new VIE-based enzymatic probes.