Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis | … Western blot analysis was performed using anti-HA-HRP antibody (Genscript, Piscataway Township, NJ) to confirm the presence and size of the protein secreted into the medium While transformed colonies showed expression of the correct size protein secreted into the medium … | Get A Quote |
The gravitropic bending in plant roots is caused by asymmetric cell elongation. This requires an asymmetric increase in cell surface and therefore plasma membrane components such as lipids, sterols, and membrane proteins. We have identified an early gravity-regulated protein in Arabidopsis thaliana root apices that binds stigmasterol and phosphoethanolamines. This root-specific protein interacts with the membrane transport protein synaptotagmin-1 and was therefore named InteractoR Of SYnaptotagmin1 (ROSY1). While interactions between ML-domain proteins with membrane transport proteins and their impact have been reported from animal cell systems, this is the first report of such an interaction in a plant system.... More