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In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Gen Comp Endocrinol. 2016-03-01;

Pablo J Sanchís-Benlloch, Josephine Nocillado, Claudia Ladisa, Joseph Aizen, Adam Miller, Michal Shpilman, Berta Levavi-Sivan, Tomer Ventura, Abigail Elizur

Products/Services Used	Details	Operation
Codon Optimization	… preference. The codon-optimised sequence was synthesised and ligated into the EcoRI-NotI sites of pPIC9K (GenScript, Piscataway, NJ, USA). Sufficient quantity of the construct plasmid DNA was generated in JM109 Escherichia coli …	Get A Quote

摘要

Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.