Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … immunoblotted with their respective antibodies. 2-6. Antibody for western blots Anti-p-Oct4(S229) antibody was made by GenScript (Piscataway, New Jersey). Cdc2 p34 (sc-54), Oct4 (sc-5279), PP1α (sc-6104) and PP1γ (sc-6108) antibodies … | Get A Quote |
Proper regulation of the cell cycle is essential to safeguard the genomic integrity of embryonic stem cells (ESCs) while maintaining the fast proliferation rate. The pluripotency factor OCT4 has been shown to inhibit CDK1 activation, thus preventing mitotic entry and facilitating the maintenance of genomic integrity. Yet, how ESCs enter mitosis in the presence of OCT4 remains unclear. We previously reported that COPS2 promotes the progression through the G2/M phase of mouse ESCs. In this study, through co-immunoprecipitation and mass spectrometric analysis, we found that COPS2 interacts with OCT4 and CDK1. We further demonstrated that COPS2 stimulates the activity of CDK1/CYCLIN B only when OCT4 is present. Con... More