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Codon Optimization | … A fusion gene encoding an N-terminal Strep (II) tag and protein WP_045143632.1 from the strain C. butyricum CWBI1009 was synthesized (GenScript) following the codon usage of E. coli and cloned into a pET11d vector (Agilent Technologies) to generate the expression … | Get A Quote |
: The search for putative enzymes that can facilitate gene editing has recently focused its attention on Argonaute proteins from prokaryotes (pAgos). Though they are structural homologues of human Argonaute protein, which uses RNA guides to interfere with RNA targets, pAgos use ssDNA guides to identify and, in many cases, cut a complementary DNA target. Thermophilic pAgos from , and have been identified and thoroughly studied, but their thermoactivity makes them of little use in mesophilic systems such as mammalian cells. : Here we search for and identify CbcAgo, a prokaryotic Argonaute protein from a mesophilic bacterium, and characterize its DNA interference activity. : CbcAgo efficiently uses 5'P-ssDNA gu... More