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Immunomodulatory activity-guided isolation and characterization of a novel polysaccharide from Atractylodis macrocephalae Koidz

Int J Biol Macromol. 2020-06-01; 
Wenyao Xue, Yue Gao, Qianwen Li, Qibin Lu, Zhengying Bian, Lei Tang, Yu Zeng, Cong Chen, Wei Guo
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Gene Synthesis … Specific RT-PCR primers that met the NCBI/Primer-BLAST criteria were synthesized by Genscript Biotech (Nanjing, China) and the sequences are as followings: β-actin, 5′-TAA GGC CAA CCG TGA AAA G-3′ and 5′-ACC AGA GGC ATA CAG GGA CA-3′; Foxp3, 5′-CCC … Get A Quote

摘要

The Rhizoma of Atractylodis macrocephala Koidz. is a traditional Chinese herbal medicine that has been widely and empirically used for unexplained recurrent spontaneous abortion. In this study, a polysaccharide from the Rhizoma of Atractylodis macrocephala Koidz., designated as RAMP2, with an absolute molecular weight of 4.354 × 10 Da was isolated and found to be composed of mannose, galacturonic acid, glucose, galactose and arabinose. The NMR results displayed that →3-β-glcp-(1→, →3,6-β-glcp-(1→, →6-β-glcp-(1→, T-β-glcp-(1→, →4-α-galpA-(1→, →4-α-galpA-6-OMe-(1→, →5-α-araf-(1→, →4,6-β-manp-(1→ and →4-β-galp-(1→ were the main linkages in RAMP2. TEM and SEM results i... More

关键词

Atractylodis macrocephala Koidz., IL-2/STAT5 pathway, Immunomodulatory