Incorporation of a Novel CD4+ Helper Epitope Identified from Aquifex aeolicus Enhances Humoral Responses Induced by DNA and Protein Vaccinations
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Operation |
| Gene Synthesis |
200,000 cells were then plated in each well in the mouse
IFNγ ELISpot plates (MabTech), followed by addition of peptide pools that span both the lumazine synthase,
3BVE, PfV or GT8 domains, or individual LS-3, LS3KO or PADRE peptides at 5ug/mL of final concentration
for each peptide (GenScript).
15-mer peptides spanning the LS and GT8 domains of eOD-GT8-60mer (GenScript) were arranged into
row and column pools (each peptide appears exactly once in the row pool and once in the column pool). |
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| Peptide Synthesis |
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