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Profiling Eph receptor expression in cells and tissues: A targeted mass spectrometry approach.

Cell Adh Migr.. 2012-03;  6(2):102-12
Noberini R, Rubio de la Torre E, Pasquale EB. 1 Sanford-Burnham Medical Research Institute; La Jolla, CA USA; 2 Department of Pathology; University of California San Diego; San Diego, CA USA.
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摘要

The Eph receptor tyrosine kinase family includes many members, which are often expressed together in various combinations and can promiscuously interact with multiple ephrin ligands, generating intricate networks of intracellular signals that control physiological and pathological processes. Knowing the entire repertoire of Eph receptors and ephrins expressed in a biological sample is important when studying their biological roles. Moreover, given the correlation between Eph receptor/ephrin expression and cancer pathogenesis, their expression patterns could serve important diagnostic and prognostic purposes. However, profiling Eph receptor and ephrin expression has been challenging. Here we describe a novel and... More

关键词

LC/MS/MS; ephrin; protein tyrosine kinase; pull-down.