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Interrogating Adaptive Immunity Using LCMV

Current Protocols. 2020-07;

Tanushree Dangi, Young Rock Chung, Nicole Palacio, Pablo Penaloza‐MacMaster

Products/Services Used	Details	Operation
Peptide Synthesis	The GP70-77 CD8 epitope is embedded within the GP61-80 CD4 epitope. Thus, stimulation of LCMV-immune splenocytes with GP61- 80 results in stimulation of both CD8 and CD4 T cells (Penaloza-MacMaster, Barber et al., 2015). All of these peptides could be custommade (e.g., through GenScript), and some may be purchased already pre-made (e.g., through Anaspec). There are also multiple CD8 epitopes identified in the Z and L proteins of LCMV.	Get A Quote

摘要

In this invited article, we explain technical aspects of the lymphocytic choriomeningitis virus (LCMV) system, providing an update of a prior contribution by Matthias von Herrath and J. Lindsay Whitton. We provide an explanation of the LCMV infection models, highlighting the importance of selecting an appropriate route and viral strain. We also describe how to quantify virus‐specific immune responses, followed by an explanation of useful transgenic systems. Specifically, our article will focus on the following protocols. © 2020 Wiley Periodicals LLC.