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Comparative study on quantitation of human myoglobin by both isotope dilution mass spectrometry and surface plasmon resonance based on calibration-free …

Analytical and Bioanalytical Chemistry. 2020-02; 
Tingting Hu, Liqing Wu, Xiaonan Sun, Ping Su & Yi Yang
Products/Services Used Details Operation
Endotoxin Detection & Removal System … Monoclonal antibodies (v50051, v50052) that recognize hMYO were purchased from GenScript Biotech Corp (Jiangsu, China). Isotopic amino acids (13C5-proline, 13C9-phenylalanine, 13C5-valine) were purchased from Cambridge Isotope Laboratory (MA, USA) … Get A Quote

摘要

The activity of proteins rather than the concentration of proteins in biopharmaceutical and in vitro diagnostics are often the primary focus. Nonetheless, development of a calibration-free concentration analysis (CFCA) approach that accurately quantifies the concentration of proteins based on molecular interactions with specific monoclonal antibodies and without the requirement of external calibrators would be beneficial to diagnostics. Generally, only analytes that interact with the antibody (Ab) are quantified by CFCA. Moreover, protein concentrations measured by CFCA usually vary when different Abs are used, and are lower than those obtained by amino acid analysis because any non-native state population of t... More

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