Products/Services Used | Details | Operation |
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Catalog Antibody | The antibodies against cpTha4 (1:10000) and cpTatC (1:5000) are described in (Mori et al., 2001). Anti-Histone H3 (1:10000) was purchased from Agrisera (www.agrisera.com).The antibody against mtTATC (1:500), pre-immune serum (1:1000) and two separate mtTATC peptides (Table 2.2) were all obtained from GenScript. | Get A Quote |
The unique Twin Arginine Translocation (Tat) pathway which is composed of TatA, TatB and TatC, transports fully folded proteins without the use of ATP energy. It was thought to be lost from the mitochondrion, the powerhouse of the cell during post-endosymbiotic evolution. This study investigates the stress induced expression of a minimal, mitochondrial Tat pathway in Arabidopsis thaliana. We treated plants with salicylic acid (SA) stress which mimics the hypersensitive response and studied the putative mitoTat components over a 24-hour time period. qRT-PCR analysis indicated the upregulation of the mitochondrial gene mtTATC. Furthermore, immunoblot detection confirmed that the mtTATC protein is expressed at h... More