Products/Services Used | Details | Operation |
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Catalog Antibody | The following primary antibodies were used: Anti-bHLH142, rabbit polyclonal antibody (Genscript) at 1:4000 dilution, Anti-EAT1 rabbit polyclonal antibody (Genscript) at 1:2000 dilution, and Anti-eGFP rabbit polyclonal antibody (Yao-Hong Biotechnology, Cat#YH-80005) at 1:10000 dilution. | Get A Quote |
Development of stable male sterility lines is essential for efficient hybrid seed production. We previously showed that knockout of bHLH142 in rice (Oryza sativa) causes pollen sterility by interrupting tapetal programmed cell death (PCD). In this study, we demonstrated that overexpression of bHLH142 (OE142) under the control of ubiquitin promoter also leads to male sterility in rice by triggering the premature onset of PCD. Protein of bHLH142 was found to accumulate specifically in the OE142 anthers. Overexpression of bHLH142 induced early expression of several key regulatory transcription factors in pollen development. In particular, the upregulation of EAT1 at the early stage of pollen development promot... More