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Proteins, Expression, Isolation and Analysis | … The protein profiles of the soluble fractions from the three cuticular structures were separated on an SDS-PAGE 4–12% Tris–glycine gel (Genscript, Piscataway, NJ, USA) using MES buffer, pH 5. The resulting bands were visualized by Coomassie brilliant blue staining, excised … | Get A Quote |
Crustacean cuticular structures are key features formed during a molt cycle. These structures are complex biomaterials comprising chitin and different mineral forms in distinct scaffold organizations. The formation of these complex biomaterials is controlled by the organic extracellular matrix including structural proteins. Since cuticular structures are formed de novo during each molt cycle, the spatial and temporal expression patterns of structural proteins are tightly linked to molt cycle events. As a model scenario, we demonstrate the molt-related pattern of expression of the gene encoding GAP65, a core structural protein involved in the formation of the cuticular structures of Cherax quadricarinatus. Based... More