| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | The protein sequence encoded by BdACT2a was sent to Genscript (Piscataway, USA) to produce a codon optimized sequence (Fig. S1) in pET28a for expression in E.coli. This construct was transformed into E. coli BL21(DE3) pLysS for expression and purification. | Get A Quote |
Plants have a variety of enzymes which help defend from pathogen attack by producing antimicrobial compounds. One of these enzymes, agmatine coumaroyl transferase (ACT) is responsible for producing hydroxycinnamic acid amides (HCAAs). In this study, we identified a tightly clustered group of ACT genes in Brachypodium distachyon responsive to Fusarium infection and functionally characterised one of these genes designated as BdACT2a. It was found that when expression of BdACT2a is disrupted, Brachypodium becomes more susceptible to Fusarium pseudograminearum. BdACT2a has broad substrate specificity and feruloylagmatine is produced in significantly lower quantities in the Bdact2a line compared ... More
