| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The SH2 domain of human Nck2 cloned into pGEX-6P1 (ref. 12) was a gift from T. Stradal (Helmholtz Centre for Infection Research). SH2 was amplified by PCR, with primers adding NcoI and NotI sites at the 5′ and 3′ ends of DNA, and subcloned into linearized pET15HE19 using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs). All other genes were synthesized commercially and subcloned into the same pET15HE bacterial expression vector (GenScript). | Get A Quote |
Protein engineering has enabled the design of molecular scaffolds that display a wide variety of sizes, shapes, symmetries and subunit compositions. Symmetric protein-based nanoparticles that display multiple protein domains can exhibit enhanced functional properties due to increased avidity and improved solution behavior and stability. Here we describe the creation and characterization of a computationally designed circular tandem repeat protein (cTRP) composed of 24 identical repeated motifs, which can display a variety of functional protein domains (cargo) at defined positions around its periphery. We demonstrate that cTRP nanoparticles can self-assemble from smaller individual subunits, can be produced from... More
