| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Later, 2.5 μl of RT product was amplified using Taq polymerase (GenScript, Piscataway, NJ, U.S.) and standard PCR conditions described previously (Quito-Avila et al. 2011), except for a varied annealing temperature according to each assay | Get A Quote |
The first search for viruses in berries (blackberry, raspberry and blueberry) was conducted in Mexico. In September 2017, 24 berry plants displaying virus-like symptoms were sampled in the main berry-producing areas of Mexico and later analyzed for the presence of 29 different viruses via enzyme linked immunosorbent assay (ELISA) and reverse transcription PCR (RT-PCR). Additionally, virus screening was verified using high throughput sequencing (HTS). As a result of this study, two blackberry plants collected in Los Reyes de Salgado, Michoacán were determined positive for tobacco ringspot virus (TRSV; genus Nepovirus), such plants exhibited yellowing and ringspot on leaves. In contrast, the rest of berry sample... More
