Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | Choline and pyruvate kinase were from Sigma. ATP, NADH, phosphoenolpyruvate, and lactate dehydrogenase were purchased from Roche. SDS-PAGE gels were purchased from GenScript, and run using the supplied MOPS bufer. CM5 sensor chip for SPR was from GE Healthcare Life Sciences and used on a Biacore T200 system. | Get A Quote |
Choline kinase alpha is a 457-residue protein that catalyzes the reaction between ATP and choline to yield ADP and phosphocholine. This metabolic action has been well studied because of choline kinase's link to cancer malignancy and poor patient prognosis. As the myriad of x-ray crystal structures available for this enzyme show, chemotherapeutic drug design has centered on stopping the catalytic activity of choline kinase and reducing the downstream metabolites it produces. Furthermore, these crystal structures only reveal the catalytic domain of the protein, residues 80-457. However, recent studies provide evidence for a non-catalytic protein-binding role for choline kinase alpha. Here, we show that choline ki... More