| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | the 5’-end flanking sequence of the U6 snRNA, which was selected as the promoter region; a 20-base sequence of the gRNAw (Figure 1—figure supplement 2A); a 76-base fragment with a gRNA scaffold; and a 3xP3-eGFP-SV40 fragment. The first three fragments were generated using commercial gene synthesis (gBLOCK by IDT and GenScript). | Get A Quote |
Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in Ae. aegypti. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wi... More
