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| Gene Synthesis | cDNAs were generated using the RevertAid™ First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA). qRT-PCR was performed using a Fast SYBR Mixture (CWBIO, Beijing, China) on a real-time thermal cycler (qTower2.2, Analytic Jena, Leipzig, Germany). ZaUBC and ZaGAPDH were selected as the reference gene [17]. Gene-specific primers were designed using the online primer-design software (http://www.genscript.com.cn/technology-support/on-line-tools), and the primers used in qRT-PCR are shown in Table S1. The 2-ΔΔCt method was used to calculate the relative expression levels of the candidate genes [18]. Three technical replicates and three biological replicates were examined for each sample. | Get A Quote |
As a traditional spicy condiment, Zanthoxylum armatum var. novemfolius is of high economical and medicinal value. Despite the long history of human cultivation, the molecular mechanisms underlying flower development are still poorly understood in Z. armatum. In this study, we performed de novo transcriptome assembly and comparative analysis of female and male flowers in Z. armatum. A total of 94,771 unigenes were obtained, and 50,605 unigenes were successfully annotated against the public database. Transcriptome data showed that 20,431 annotated unigenes were differentially expressed genes. KEGG enrichment analysis revealed that the most representative pathway was plant hormone signal transduction. Amon... More
