| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Arginine modified-glutathione peptides (SG-1Arg = C16H29N7O7S(CF3COO−); SG-2Arg = C22H40N11O8S (CF3COO−)2; SG-3Arg = C28H53N15O9S(CF3COO−)3 with 10% of H2O and purity ≥ 95%) were purchased from GenScript (USA). Gold(III) chloride trihydrate (HAuCl4·3H2O, reagent grade), reduced L-glutathione (≥98%), sodium borohydride (NaBH4), isopropyl alcohol (IPA, 99%) were purchased from Sigma-Aldrich (France). Sodium hydroxide (NaOH, 98%) and hydrochloric acid (HCl, 35–37%), were purchased from Laurylab and VWR Chemicals BAH. Formvar carbon grids were purchased from Agar scientific. Phosphate buffer (PBS, 10 mM) and cell culture medium (DMEM, Dulbecco's Modified Eagle's medium) were purchased from Gibco (Thermofisher, France). All products were used as received without further purification. Water was purified using a Millipore Milli-Q system (Millipore, France) for all experiments. | Get A Quote |
A library of ultra-small red photoluminescent gold nanoclusters (Au NCs) were synthesized with an increasing amount of positive charges provided by the addition of mono-, di- or trivalent-glutathione modified arginine peptides. We then studied how the arginine content impacted on the interaction of Au NCs with negatively charged artificial lipid bilayers and cell membranes. Results indicated that increasing the arginine content enhanced Au NCs' adsorption on lipid bilayers and on cell membranes followed by an increased cellular uptake in melanoma cells (COLO 829). Surprisingly, the presence of up to 40% serum for highly positively charged Au NCs did not hinder their interaction with lipid bilayers that contain ... More
