| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | Para a análise dos soros, utilizou-se um peptídeo recombinante (NcGRA1), desenhado com base na metodologia de genética reversa de epítopos antigênicos originados de uma proteína dos grânulos densos de Neospora caninum e sintetizado pela GenScript (USA). Foi produzido por meio de processo fermentativo em leveduras Pichia pastorisKM71 no mesmo local (LBCHV/DVT/Bioagro), na Universidade Federal de Viçosa. | Get A Quote |
The neosporosis has great importance in cattle, and its pathogenesis in this host has been generally clarified, however, in horses, neosporosis is poorly known, and in Colombia anti-Neospora spp antibodies have not been reported. Therefore, the main objective in the present study was to evaluate the occurrence of this parasite in donkeys (Equus asinus) from farms that presented a risk of disease in these animals, as well as no health plan for them. Were used 56 animals randomly chosen inside selected farms in the state of Sucre (Colombia). A recombinant peptide originated from Neospora caninum (NcGRA1) was used for the diagnosis with Dot-ELISA and serum was diluted 1:200. This study is the first to report the... More
