Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | The purified proteins were subjected to SDS-PAGE separation and transferred to nitrocellulose membranes using a semidry transfer unit (Bio-Rad). After 10 min incubation with Rapidblock solution (VWR), primary antibodies against GFP or Histone H3 (Genscript, Piscataway, NJ) were used at 1:2,000 dilution for overnight incubation at 4°C. | Get A Quote |
Understanding the regulation of gene expression, from the epigenetic modifications on genomes to posttranscriptional and translational controls, are critical for elucidating molecular mechanisms underlying distinct phenotypes in biology. With the rapid development of Multi-Omics analyses, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on Tri Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitati... More