| Products/Services Used | Details | Operation |
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| Peptide Synthesis | A 26 amino acid sequence corresponding to a SerThr-Tyr domain within TEAD4216 (Figure 1), linked to a 10 amino acid cell importation signal (RMR) was synthesized (GenScript NJ). Human ocular melanoma cells (Mel 270, Mel 202; a kind gift from Dr Bruce Ksander), retinoblastoma cells (Y79; ATTC, MD), primate retina/choroid ocular endothelial cells (RF/6A; ATCC, MD) and human retinal pigment epithelial cells (ARPE19; ATCC, MD), (CRL 1500 breast cancer cell line; ATCC, MD) were plated into 96 well plates and cultured for 24 h. Recombinant STY-RMR peptide was added to the cell culture media at various concentrations (10 to 30 mg /100ml). | Get A Quote |
Purpose: Transcriptional enhancer factor 1-related (RTEF-1) also known as TEAD4 is expressed in ocular vascular endothelial cells and plays a role in the control of VEGF expression. Alternative processing of TEAD4 hnRNA results in different proteins able to stimulate or inhibit VEGF gene transcription. The purpose of this study is to test whether short peptide fragments (STY-RMR), representing functional domains of the inhibitory TEAD4216 isoform, can inhibit tumor as well as endothelial cell proliferation. Experimental Design: Cell proliferation was assessed using a colorimetric assay in cell lines incubated with STY-RMR, the amount of secreted VEGF within media was determined both in treated and control cell ... More
