| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | Plasma LPS concentration was determined using the Toxin Sensor Chromogenic LAL Endotoxin Assay Kit (GenScript, Piscataway, NJ, USA). All plasma samples were extracted with chloroform prior to the LAL assay as described by Harris et al. (1990). Briefly, the diluted plasma was mixed in a ratio of 1:1 (v/v) with chloroform and incubated for 1 hour in a thermostat block (25°C) with shaking at 800 rpm. Then the emulsion was separated by centrifugation at 2,000 g for 20 min and the top aqueous layer was collected for endotoxin assay. The appropriate dilution factors were determined previously to make sure the measurements fall in the range of the standard curve. Measurements of TNF-α concentration in plasma were performed using porcine TNF-α Elisa kit (Thermo Scientific Pierce, Rockford, IL, USA) according to the manufacturer’s instructions. | Get A Quote |
The uneven development of adipose tissues reflects a differential occurrence of biological events in vivo while the underlying molecular mechanism remains largely unknown. In the present study, the in vivo inflammatory status of an inbred obese porcine model, Lantang pig, was assessed, aiming to provide evidence for obesity biology. Compared with genetically lean pigs (crossbred, Duroc × Landrace × Large White), Lantang pigs exhibited a larger amount of ultra large adipocytes in subcutaneous adipose tissue accompanied with higher expression of macrophage/monocytes markers and pro-inflammatory genes (TLR4, CD14, CD11β, MCP1, TNFα, IL1β and IL6) and lower expression of cellular antioxidant enzymes (SOD1, 2 a... More
