| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | NCBI (https://www.ncbi.nlm.nih.gov/) and the resulting hits were filtered for presence of redundant sequences and the biophysical properties and the subcellular localizations were determined using ProtParam tool (https://web.expasy.org/protparam/) and WoLF PSORT (http://www.genscript.com/wolf-psort.html) respectively. | Get A Quote |
Tef [Eragrostis tef (Zucc.) Trotter] is becoming popular for its healthy diet and resilience to changes in climate. TFs are genes regulating in the transcription through binding to specific sequences or by protein-protein interaction. Dofs are plant specific TFs and involved in different plant specific processes. Little is known about their features in tef and comparative phylogenetic relationship of Dof proteins with other plants. In silico identification is a popular method to identify functional genes in almost every plant. Thirty three (33) Dof TFs have been identified through BLAST search in the available databases. Multiple sequence alignment and phylogenetic tree were constructed using MEGA X soft... More
