| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | The peptide H-Orn-Orn-Trp-Trp-NH2 (H-OOWW-NH2, O3TR) and a lauric acid ester of O3TR, the lipopeptide (C12H23O)-OOWW-NH2 (C12O3TR) at >90% purity were purchased from GenScript Corporation, Nanjing, China. The purity was selected based on cost consideration and other relevant studies (Bisht et al., 2007; Badosa et al., 2009; Laverty et al., 2015; Thery et al., 2018). Peptides were synthesized by solid-phase methods using N-(9-fluorenyl) methoxycarbonvl (Fmoc) chemistry. Peptides O3TR and C12O3TR were amidated at the C terminus (NH2). The peptides were reconstituted at 1 mmol L−1 in sterile distilled water and stored in vials at −40 °C. | Get A Quote |
The present study evaluated the antifungal properties of tetrapeptide H-OOWW-NH2 (O3TR) and its derivative lipopeptide C12-OOWW-NH2 (C12O3TR) against Penicillium digitatum, one of the main postharvest pathogens in citrus, and the possible mechanisms of their antifungal action. The results showed that the peptides O3TR and C12O3TR could inhibit conidial germination, induce conidia death and reduce the survival of mycelia of P. digitatum in vitro. The antifungal properties of O3TR and C12O3TR against P. digitatum were thermostable (40 °C–80 °C), insensitive to the change of pH (3–10) and varying sensitive to the presence of cations (Na+, Ca2+). In addition, the two peptides could effectively contr... More
