Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | The chicken breasts were purchased 36 h postmortem from local market (Sushi Food Co., Ltd., Nanjing, China), and were stored under −20 °C till required (within one week). Bovine serum albumin (BSA), β-mercaptoethanol (β-ME), 8-Anilino-1-naphthalenesulfonic acid (ANS), 5,5′-Dithiobis-(2-nitrobenzoic acid) (DTNB) and phosphotungstic acid was provided by Sigma (Sigma Aldrich, St. Louis, MO, USA). Protein maker and 4–12 g/mL gel of SDS-PAGE was provided by Genscript (Genscript Co., Ltd., Nanjing, China). | Get A Quote |
High pressure homogenization (HPH) can solubilize myofibrillar proteins (MPs) in water. To elucidate the effect of the HPH disruption chamber geometry on the physicochemical and structural properties of MPs in water, two types of nozzle and a counter flow interaction chamber (cf) were applied to homogenize MPs aqueous solutions at pressures of 0 MPa, 103 MPa and 172 MPa HPH for 2 passes, and the physical dispersion and conformational characteristics of MPs in water were investigated. The nozzles and counter flow chamber played a major role in the solubility of MPs in water at 103 MPa and 172 MPa HPH, respectively. Turbulence and cavitation in a narrow nozzle and counter flow action resulted in a decre... More