| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Insulin from bovine albumin was purchased from Sigma Aldrich and used without further purification. Amyloid fibrils were prepared by dissolving 0.35 mM of monomeric protein in pH2 (HCl solution) and aggregating at 65 °C for 24 h. Insoluble aggregates were centrifuged at 775 × g using microcentrifuge 5418 and the supernatant was taken for further experiments. LVEALYL peptide was purchased from Genscript and used as obtained. LVEALYL:insulin fibrils were prepared by mixing LVEALYL peptide (1.95 mM) with insulin monomer in molar ratios 1:20 and 1:40 following the same aggregation procedure as for the insulin fibrils. | Get A Quote |
Amyloid fibrils are a hallmark of neurodegeneration. The structural diversity of amyloids necessitates sensitive methods and probes that can be reliably used to characterize them. Here, we study insulin fibrils and its polymorphs seeded with LVEALYL peptide in context of probing the surface patterns using Thioflavin T (ThT) and polythiophene derivative – Poly[2-(3-thienyl)ethoxy-4-butylsulfonate] (PTEBS) polymer. We investigated the dynamics and lifetimes of these two probes using time-resolved absorption and fluorescence spectroscopy. The photoluminescence emission lifetimes of the probes showed different relaxation times in the presence of structurally different amyloid fibrils. However, only PTEB... More
