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| Catalog Antibody | Chromosomal DNA from each isolate was extracted from bacterial cells using Genomaker® (Bio-East Technology, Taiwan). The isolated DNA was qualified at an A260/A280 ratio of 1.8–2.0 and quantified using Nanophotometer (Implen, Germany). The PCR conditions were as follows: initial denaturation at 95 °C for 10 min followed by 32 cycles consisting of 95 °C for 30 s, 48 °C for 30 s, 72 °C for 30 s; and a final extension at 72 °C for 5 min. PCR products were resolved by electrophoresis on 2% agarose gels in 0.5 × TBE buffer (1 mM Tris, 0.01 M EDTA, and 1 M boric acid). The gels were stained with DNAsafe™ Nucleic Acid Gel Stain II (GenScript, USA) and then visualized for photography under UV light using a Gel Doc™ XR + (Bio-rad). | Get A Quote |
Lancefield group C Streptococcus dysgalactiae represents an etiological agent causing high mortality in many farmed fish species worldwide. Despite its pathogenic importance, limited epidemiological knowledge related to this pathogen is available. In the present study, 79 S. dysgalactiae isolates from diseased aquatic animals in Taiwan (n = 67) and Japan (n = 12) were characterized using pulsed-field gel electrophoresis (PFGE). The distribution of isolate virulence factors and antimicrobial susceptibility were also investigated. PFGE with SmaI and ApaI digestions displayed 19 and 20 different pulsotypes respectively, reflecting a genetic diversity among isolates from different sources. All examined... More
