| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Peptides (Table 1) and HLA-A*11:01-restricted KRAS G12V8-16 (VVGAVGVGK) as positive peptide were synthesized from GenScript (Nanjing, China), with purity greater than 98% by mass spectroscopy. Peptide-MHC tetramers were generated as previously described [23]. In briefly, Peptides (400 μM) were mixed with Flex-T™ HLA-A*11:01 Monomer UVX (Biolegend), then subjected to UV light for 30 min on ice. The MHC monomers exchanged with peptides were tetramerized in the presence of allophycocyanin (APC) conjugated streptavidin (BD Biosciences) for 30 min at 37 °C, then the reaction was stopped by PBS containing D-Biotin (500 μM) and NaN3 (10%), then kept at 4 °C overnight for use. | Get A Quote |
Colorectal cancer (CRC) is a malignant cancer with high incidence and mortality in the world, as the result of the traditional treatments. Immunotherapy targeting neoantigens can induce durable tumor regression in cancer patients, but is almost limited to individual treatment, resulting from the unique neoantigens. Many shared oncogenic mutations are detected, but whether the common neoantigens can be identified in CRC is unknown. Using the somatic mutations data from 321 CRC patients combined with a filter standard and 7 predicted algorithms, we screened and obtained 25 HLA-A*11:01 restricted common neoantigens with high binding affinity (IC50<50 nM) and presentation score (EPIC>0.9). Except the positive epito... More
