| Products/Services Used | Details | Operation |
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| Peptide Synthesis | NOP-1 peptides (GenScript, Piscataway, NJ, United States) were dissolved in a FRET-buffer modified after Bisson et al. (2016) (50 mM Tris−HCl pH 8.0, 300 mM NaCl, 0.015% (w/v) Fos-Choline-16, 0.05% (v/v) Tween 20). On each fruit, a total of 200 microdroplets (0.5 μL each) were applied with a Hamilton microdispenser (Hamilton Bonduz AG, Bonduz, Switzerland) over the whole tomato surface. Based on previous dose-finding studies on tomatoes, a NOP-1 concentration of 1000 μM was used (Kessenbrock et al., 2017). A concentration of 1000 nL L-1 (1.6 g/m3 of SmartFreshSM 0.14% active substance) was used for the 1-MCP treatment (SmartFresh, Agrofresh Milano, Italy), according to the standard recommendations of the manufacturer. Untreated fruits served as control treatment. After application of the ripening inhibitory substances, each treatment was allocated in a storage box and kept at room temperature (19 ± 2 °C) for 21 days. | Get A Quote |
The synthetic octapeptide NOP-1 inhibits ethylene downstream signalling by disrupting the ETR1- EIN2 interaction, resulting in ripening delay of tomatoes when applied at early ripening stages. In order to better understand the NOP-1 induced ripening delay, we investigated the effect of a repeated surface application of NOP-1 as well as its application at advanced maturity stages of tomatoes. For this, analytic and non-destructive evaluation parameters were used. NOP-1 delayed ripening when applied at the maturity stage breakers (BR) as indicated by colour development. Repeated application had no additional delaying effect and did not reach the level of ripening delay induced by 1-MCP. When applied at maturity ... More
