| Products/Services Used | Details | Operation |
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| Peptide Synthesis | Bovine DCD peptide was synthesized by FlexPeptide™ Technology (GenScript, USA). The antimicrobial activity of Bovine DCD was analyzed as described by Valore et al. [20] using Gram-positive bacteria included Enterococcus faecalis (ATCC29212), Staphylococcus epidermidis (ATCC1228), methicillin-resistant Staphylococcus aureus (MRSA) (ATCC43330), Streptococcus bovis (ATCC49147); while Gram-negative bacteria included Escherichia coli (ATCC25922), and the fungus Candida albicans (ATCC1021). E. coli was grown in LB medium, S. epidermidis in Nutrient medium, S. bovis in Trypticase soy medium with defibrinated sheep blood, E. faecalis and S. aureus in Columbia medium, and C. albicans in Casein hydrolysate medium. The bacterial and yeast concentrations were determined photometrically. Bacteria and yeast were diluted to a concentration of 2X106 CFU/ml in phosphate buffer. The cells were incubated at 37ºC for 4 h with different peptide concentrations or sweat fractions in 200 µl of phosphate buffer. | Get A Quote |
Description of a novel bovine antimicrobial peptide and its antimicrobial spectrum. RNA isolation and RT-PCR were done from various tissues. DCD peptide was synthesized, and antimicrobial activity was analyzed. Bovine dermcidin gene contains five exons intermittent by 4 introns. Bovine DCD-mRNA was 398 bp with ORF 336 bp. Bovine DCD was expressed in skin and blood. Analysis of the amino acid compositions revealed that cysteine was repeated 6 times indicating the presence of 3 disulfide bonds that play role in the peptide stability. Staphylococcus epidermidis, Streptococcus bovis, and Enterococcus faecalis were affected by Bovine DCD peptide. Highest antimicrobial effect was at 50 and 100 µg/ml. The effect on E... More
