| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Microfluidic devices were primed with light mineral oil (Sigma) containing 2% SPAN80 (Sigma), which was also used as the continuous phase fluid. The crosslinker emulsion was comprised of 30 mg mL−1dithiothreitol (DTT; Sigma) emulsified at a 1:15 ratio in the continuous phase. The macromer solution consisted of 6.5% (w/v) PEG-4MAL (20 kDa, Laysan Bio, Arab, AL, USA), which had been reacted with a cell adhesive peptide (1.0 mM, Genscript, Piscataway, NJ, USA) that was pre-conjugated to AlexaFluor594 (Thermo Fisher, Waltham, MA, USA) to enable imaging and for consistency with cell encapsulation experiments. | Get A Quote |
Cells can be microencapsulated in synthetic hydrogel microspheres (microgels) using droplet microfluidics, but microfluidic devices with a single droplet generating geometry have limited throughput, especially as microgel diameter decreases. Here we demonstrate microencapsulation of human mesenchymal stem cells (hMSCs) in small (<100 μm diameter) microgels utilizing parallel droplet generators on a two-layer elastomer device, which has 600% increased throughput vs. single-nozzle devices. Distribution of microgel diameters were compared between products of parallel vs. single-nozzle configurations for two square nozzle widths, 35 and 100 μm. Microgels produced on parallel nozzles were equivalent to those p... More
