| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Peptide PAF56 (GHRKKWFW) was synthesized at >90% purity from GenScript Corporation (Nanjing, China) by solid-phase methods using N-(9-fluorenyl) methoxycarbonyl (Fmoc) chemistry. Stock solutions of peptides were prepared at 1 mM in sterile ultrapure water and stored at −40 °C. | Get A Quote |
The ability of the short cationic antimicrobial peptide PAF56 (amino acid sequence GHRKKWFW) to control postharvest infectious diseases of citrus fruits was evaluated. PAF56 inhibited the growth of Penicillium digitatum, Penicillium italicumand Geotrichum candidum. The minimum inhibitory concentrations (MICs) of PAF56 for the control of P. digitatum, P. italicum and G. candidum were 8, 16, and 8 μM, respectively. The conidia of P. digitatum, P. italicum and G. candidum were also highly sensitive to PAF56. The peptide PAF56 could effectively control green mold, blue mold and sour rot on inoculated citrus fruits. Fluorescence microscopy with the fluorescent stains SYTOX Green (SG) and Calcofluor Whi... More
