| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Pro-Asp-(Phe-Asp)5-Pro (denoted FD, Mw of 1638.67), Pro-Asp-(Phe-Asp)5-Gly-Gly-Gly-Arg-Gly-Asp-Ser-Pro (denoted FD-RGD, Mw of 2225.2) and rhodamine-B N-termini labelled FD were custom synthesized and then purified by high performance liquid chromatography to 95% purity (FD by American Peptide, Sunnyvale, CA, FD-RGD by Genscript, NJ, USA and labelled FD by Caslo, Lyngby, Denmark). Unless otherwise specified, all reagents were purchased from Sigma-Aldrich (Rehovot, Israel) and were of the highest available purity. | Get A Quote |
The interest in developing functional biomaterials based on designed peptides has been increasing in recent years. The amphiphilic and anionic β-sheet peptide Pro-Asp-(Phe-Asp)5-Pro, denoted FD, was previously shown to assemble into a hydrogel that induces adsorption of calcium and phosphate ions and formation of the bone mineral hydroxyapatite. In this study the integrin binding peptide, Arg-Gly-Asp (RGD), was incorporated into the hydrogel to assess its influence on an osteoblast culture. In solutions and in hydrogels FD fibrils dominated the assembly structures for up to 25 mol% FD-RGD incorporation. The cellular density of osteoblasts cultured in hydrogels composed of 25 mol% FD-RGD in FD was higher than t... More
