| Products/Services Used | Details | Operation |
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| Peptide Synthesis | The p60 protein is a surface protein involved in the growth of all Listeria species [24]. It is suitable for the detection of L. monocytogenes due to the presence of a short sequence of eleven amino acid called peptide PepD (QQQTAPKAPTE) unique to the p60 protein of L. monocytogenes (accession number AEO02672 in the NCBI protein database) [24]. After being synthetized (GenScript Inc., Piscataway, NJ, USA), the peptide was used for the production of the specific mouse anti-pepD monoclonal antibody protein of L. monocytogenes p60 protein (anti-PepD mAb) (GenScript Inc., Piscataway, NJ, USA). The specificity of detection against PepD in L. monocytogenesp60 protein using anti-PepD mAb has been investigated by Coutu et al. [25] and Etty et al. [26]. | Get A Quote |
This paper presents the development of a support membrane based on chitosan, cellulose nanocrystals and glycerol (m-CCG) for the antibody immobilization by a covalent crosslinking using glutaraldehyde. The chemical characterization of the support by FTIR showed that m-CCG formation process was stabilized by the formation of hydrogen bonding between each component of m-CCG and the reactive amine groups allowing the antibody immobilization on m-CCG via glutaraldehyde. Moreover, this immobilization on m-CCG was optimized by mathematics modeling approaches, and it exhibited robustness and predictable detection in presence of 0.6% of cellulose nanocrystals (CNCs), 0.5 g of CCG solution per well, after 2 h of ant... More
