| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | To investigate the change of BcHHP1 expression under diverse stress treatments, we performed real-time qPCR. The primers were designed by the online primer design tool from Genscript Biotechnology Company (https://www.genscript.com/ssl-bin/app/primer) and listed in Table 2 Suppl. The five-leaf stage seedlings were utilized after cold, salt, ABA, and SA treatments for 0, 0.5, 1, 2, 4, 8, and 24 h. Total RNA was extracted from the frozen plant tissues with an RNA extraction kit (Tiangen, Beijing, China). Genomic DNA contamination was removed using DNase I (Takara). | Get A Quote |
Heptahelical protein (HHP) signalling pathway is involved in cold acclimation responses to low temperature and other stresses. The HHP transcription factor family is the key component regulating this signalling pathway. In this study, five HHP-like genes, BcHHP1, BcHHP2, BcHHP3, BcHHP4, and BcHHP5, were isolated from non-heading Chinese cabbage (Brassica rapa ssp. chinensis cv. Suzhouqing). Multiple sequence alignment and phylogenetic analysis showed that BcHHP proteins are highly homologous to HHP proteins from Arabidopsis thaliana, Glycine max, Oryza sativa, and Zea mays. Some of these HHP proteins might share similar functions in some aspects, which might be further proved by interaction network of BcHHP gen... More
