| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Polyclonal antibodies for GcFBPA and GcGALE were ordered from GenScript (USA, New York). The GcFBPA and GcGALE peptides for the production of polyclonal antibodies were designed using GenScript custom peptide synthesis service (GenScript, USA, New York) by OptimumAntigen™ Design Tool (GenScript, USA, New York) (Supplementary Table 2). The selection of peptides were conducted based on the following criteria: 1) The peptide size is >4 kDa with 10–15 amino acid residues; 2) The peptide location is near to C or N terminus; 3) The peptide has high score in hydrophilicity (>0), antigenicity (>1.0), surface probability (>0); and disordered score; and 4) The peptide has the lowest similarity (<90%) to the host which is Oryctolagus cuniculus (rabbit). | Get A Quote |
The supply of agar as an important gelling and thickening agent in various industrial applications depends heavily on harvesting of natural seaweed resources and seaweed farming. To facilitate the selection of good seaweed source with higher agar yield and stronger gel strength, accurate and rapid screening method using molecular markers is necessary to replace the tedious, laborious and time-consuming conventional method which involves agar extraction and gel analysis. In this study, we characterized the expression of a number of algal transcripts and proteins from an agar producing seaweed, Gracilaria changii with the aim to identify potential markers for agar yield and gel strength. In total, 15 candidate ... More
