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Proteins, Expression, Isolation and Analysis | … the fusion protein of the functional domain of BmARM-like protein at an OD600 of 05 by adding isopropyl thiogalactoside (IPTG; final concentration 02 mM after optimization) at 16 °C for 24 h and then purified using the high affinity Ni-NTA resin (GenScript, China) according to … | Get A Quote |
Armadillo (ARM)-repeat protein is involved in many biological processes, including cell–cell adhesion and the Wnt signaling pathway. However, the function of ARM-repeat protein in Bombyx mori has not been completely clarified. In this study, a gene encoding ARM-repeat protein was identified, which has an open reading fragment of 1923 bp, encoding a predicted 641 amino acid residues with a molecular weight of approximately 71.2 kDa. BmARM-like mRNA showed highest expression in hemolymph, significantly high expression levels in 4th instar, moth, and egg, stable high expression levels in the early embryonic development, relatively high expression levels in the 1st day and 5th day of the pupa stage, and hi... More