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Proteins, Expression, Isolation and Analysis | … The lengths of the resolving and stacking gels are 12 and 3 cm, respectively, with a gel thickness of 1.5 mm. Molecular weight markers (30–150 kDa) were purchased from GenScript Corp. (Piscataway, NJ). Statistical Analysis. All experiments were replicated at least three times … | Get A Quote |
The effects of limited enzymatic hydrolysis with pepsin on the functional properties and molecular characteristics of cowpea protein concentrate (CPC) were investigated. Hydrolysates were prepared at degrees of hydrolysis (DH) 5–30%. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis demonstrated that globulin was the major protein component of CPC, and enzymatic breakdown of polypeptides was selective. As a result, significant changes in the functional properties of hydrolysates were noticed as compared to the protein concentrate. Then, cowpea protein hydrolysates (CPHs) showed good water solubility index at pH 7 (>95%) and pH 4.5 (>55%), at DH 20 and 30%, respectively. Oil‐holding ca... More