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| Codon Optimization | Using GenScript software, the codon adaptation index (CAI) was estimated for the designed sequence to ensure optimal expression in the BES. The CAI was estimated to be 0.72, a reliable index for proper expression. Finally, the designed sequence was synthesized. PCR and DNA sequencing analyses were performed to confirm the recombinant pFastBac1-Hepc25 vector. Lane 1 in Figure 1A shows the 174 bp band corresponding to the designed coding sequence. For further confirmation, DNA sequencing using universal M13 Primers was performed. | Get A Quote |
Hepcidin is the primary regulatory hormone responsible for lowering the iron content in the blood circulation. Due to its biodegradability and low cytotoxicity, hepcidin is considered as an alternative for iron chelators. The baculovirus expression system may be suitable for human hepcidin production because the expressed proteins generally exhibit proper folding, post-translational modifications, and oligomerization. Using data from two vector maps, pFastBac1 and pFastBac HTB, a unique vector was designed encoding human hepcidin-25 as fusion recombinant peptide. Expression analysis showed that it was expressed as a peptide with a molecular weight near to 5 kDa. After purification and TEV treatment, findings re... More
